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Megabace 1000 dna sequencer analyzer profiler genotyper
GE MegaBACE 1000 DNA Sequencer removed from working laboratory that upgraded to a newer model. Unit is in excellent cosmetic condition and looks almost new.
* MegaBACE 1000 main instrument
* Electrical connections and cables
* Owner s manual (MegaMANUAL-Guide to sequencing with the MegaBACE 1000).
MegaBACE 1000 DNA Analysis System is a high-throughput, fluorescence-based DNA system utilizing capillary electrophoresis with up to 96 capillaries operating in parallel. The system performs sample injection, gel matrix replacement, DNA separation, detection, and data analysis, offering unparralleled flexibility.
MegaBACE Long Read Matrix is optimized for both sequencing and genotyping applications. This saves time when switching between applications, and results in less waste.
MegaBACE Long Read Matrix can produce DNA sequences of 700 bases or resolve multiplexed fragments in excess of 900 bases. The gel matrix is replaceable, and each pack includes 96 pre-dispensed tubes plus running buffer.
All MegaBACE DNA sequencing reagent kits offer unparalleled performance on a broad range of templates:
DYEnamic ET Dye Terminators or DYEnamic ET Primers
DYEnamic ET Primer Reagents
To complement instrument functionality, we offer a range of MegaBACE ET Size Standards for a variety of applications. These include MegaBACE ET400-R, ET550-R, and ET900-R.
For SNP validation, MegaBACE SNuPe Genotyping Kit contains everything needed to perform a robust single-base extension reaction of PCR products in a single-tube format.
Software The following software tools are available:
Instrument Control Manager is used for instrument set-up and sample entry.
Sequence Analyzer processes large amounts of data without manual intervention.
MegaBACE Sequencing ScoreCard offers a time-saving overview of all samples.
MegaBACE Fragment Profiler allows the user to automate the analysis of di-, tri-, and tetranucleotide repeats of polyploid organisms. User optimized peak filters allow for the automated analysis of atypical peak patterns.
MegaBACE Genetic Profiler is optimized for diploid dinucleotide repeat analysis.
MegaBACE Genetic Profiler ScoreCard allows quality comparisons between capillaries and runs.
MegaBACE SNP Profiler saves editing time by assigning quality scores to each SNP call.
Operating system: Windows 2000
Software: Microsoft Office Pro
Sequencing read length and throughput
With the ability to sequence 96 samples in just 2 hours and perform up to 9 runs per day, MegaBACE 1000 is the ideal choice for production-scale sequencing projects. Average read length with dye primer chemistry is 550 bp at 98.5% accuracy with M 13 standard template. With a slower, 3-hour run time, sequences of up to 1000 bases is possible. MegaBACE 1000 can produce more than 500 samples per 9-hour day.
Genotyping sizing precision, accuracy, and throughput
The MegaBACE DNA Genotyping System automatically processes 2000 microsatellite genotypes per 96-well run. The total turnaround time per run is less than 2 hours from injection to injection. This corresponds to more than 14 000 genotypes per system per day. For SNP analysis, MegaBACE 1000 can deliver >5 760 SNPs per day.
Minimal sample loading volume of 5 l. The samples are introduced to the system in a 96-well format. Samples can be reinjected up to 10 times.
Single mode (488 nm) for sequencing
Dual mode (488 and 532 nm) for genotyping
Confocal optics with multiple filter sets
Digital resolution is 16 bits linear to 5%
Computer and software not included. Software available through GeneTool, Inc. for $1000 that includes MEGABACE 1000.ICM v3.1 (to run the instrument) and Sequencing Software Suite v3.0 (for data analysis).
